Macquarie University Microscopy Unit
Faculty of Science

 

 

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Transmission Electron Microscopy

Scanning Electron Microscopy

Laser Scanning Confocal Microscopy

Light Microscopy and digital image capture

Histology

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OLYMPUS FLUOVIEW FV1000 IX81 INVERTED CONFOCAL MICROSCOPE

The Olympus Fluoview FV1000 is a versatile point-scanning, point-detection, confocal laser scanning microscope suitable for various research applications and is ideal for 3-D imaging, time course experiments, energy transfer visualisation and photobleaching recovery experiments. It delivers the high resolution required for multi-dimensional observation of cell and tissue morphology as well as precise molecular localisation.  The system allows for real-time visualization of rapid cell responses and is able to measure diffusion coefficients of intracellular molecules, quantifying molecular kinetics. The FV 1000 is equipped with 4 laser diodes (405, 473, 559 and 635). This allows for collection on 3 fluorescence channels, 1 transmitted light channel and sequential multi-channel acquisition of up to 10 channels.

 

LEICA VT 1000S VIBRATOME

The VT 1000s is a vibrating blade microtome that can produce sections of fresh tissues as thin as 20μm that permit enhanced optical resolution. The combined use of vibratome sections and the inverted microscope confocal system allows for imaging experiments to be performed with the high numerical aperture immersion objectives. The vibratome facilitates experimental work where cellular visualization is critical.